Glycogen synthase activity and glycogen content in muscle and liver of GSK-3 liver KO animals.

<p>Glycogen synthase activity was determined in (A) liver and (B) quad of eight weeks old Alb Cre – (open bars) and Alb Cre + (filled bars) tissues by assaying incorporation of glucose from uridine diphospho-(6-³H)-D-glucose into glycogen and expressed as a ratio of activity in the absence divided by that in the presence of G-6-P. Values are the mean ± SEM for four separate experiments with each assayed in duplicate. Glycogen content was measured in liver (C) and quad (D) liver from eight week old ALB Cre − (open bars) and ALB Cre + (filled bars) following either an overnight fast or random feeding. Values are mean ± SEM from eight separate animals with each assayed in triplicate. For these experiments, the mouse background strain was C57BL/6/129.