Commercial MAb, 0211 cross-reacts with dsDNA.

<p>(A) MAb 0211 binds to EBNA-1 as detected by ELISA but not P2, or BSA. (B) MAb 0211 binds to dsDNA as detected by ELISA. (C & D) MAb 0211 was adsorbed onto dsDNA cellulose beads and pre and post adsorbed antibody were tested for binding to dsDNA and EBNA-1 by ELISA. (C) MAb 0211 adsorbed on dsDNA cellulose beads, was completely depleted of antibody reactivity for dsDNA and EBNA-1. Anti-dsDNA and anti-EBNA-1 ELISAs were performed on separate ELISA plates and ELISAs were developed when ODs reached maximal values. Results represent OD 405 nm values after subtraction of non specific binding to cellulose only beads. (D) Post dsDNA cellulose adsorbed MAb 0211 shows reduced binding to rEBNA-1 by Western blot. Left panel: Coomassie stained polyacrylamide gel. Right panel: Western blot: filters were immunostained with pre (lanes 2 and 3) or post dsDNA cellulose adsorbed 0211 (lane 4) as indicated.