3D4 binds to the carboxyl region while 0211 binds to both the carboxyl and amino regions of EBNA-1.

<p>(A) Functional map of the complete EBNA-1 protein containing the Gly-Ala repeat region (GA). CBS 1,2,3; chromatin binding sites, NLS; nuclear localization site, VBS: viral binding site. LS8 denotes the amino fragment (aa 1–404) lacking most of the GA repeat. LS7 denotes the amino fragment (aa 1–393) lacking the GA repeat and lacking PPPGRRP. LS9 denotes the carboxyl fragment (aa 410–641). (B) MAb 3D4 is strongly reactive with LS9 but not LS8 or LS7 as detected by ELISA. (C) MAb 0211 is reactive with LS7, LS8 and LS9 as detected by ELISA. Results in B and C are the average of triplicate wells.