Constructs of TDP-43 fragments.

<p>A schematic illustration of various constructs of human TDP-43 fragments. The protein domains are marked on the top. RRM means RNA recognition motif. GRD means glycine-rich domain. The structure is not drawn in proportion. The amino acid numbers are marked for each mutant. The red segment in construct 4 marks the C-terminal half of the RRM2 that is necessary for aggregation (see text). For construct 8, the GRD was deleted and then the two fragments were joined together. EGFP was fused to the C-terminus of all the TDP-43 constructs.