Expression of AMP genes in crayfish hepatopancreas and hemolymph after bacterial challenge.

<p>Hepatopancreas and hemolymph were taken out from the crayfish at 6 h after bacterial injection. Five hundred and 100 ng of total RNA from hepatopancreas and hemocytes, respectively, were used for RT-PCR. The PCR product of each gene was detected at the following cycles: 25 cycles for 40S; 30 and 25 cycles for <i>Pl</i>crustin1 in hepatopancreas and hemocyte, respectively; 28 and 22 cycles for <i>Pl</i>crustin2 in hepatopancreas and hemocyte, respectively; 30 cycles for <i>Pl</i>crustin3, LGBP, ALF and astacidin2. Each lane shows gene expression in each crayfish.