Aβ inhibits NO-induced cGMP synthesis and function.

<p><b>A</b> BAEC were pretreated with 10 µM Aβ followed by 10 µM DEA/NO. <b>B</b> HUVEC were pretreated with 10 µM Aβ followed by 90 µM IBMX and 10 µM DEA/NO. <b>C</b> Jurkat cells were pretreated with 10 µM Aβ followed by an NO-independent sGC activator (BAY 41-2272). <b>D</b> Jurkat cells were pretreated with Aβ (0.1–100 µM) followed by 1 µM DEA/NO. <b>E</b> Porcine VSMC were pretreated with Aβ (0.1–10 µM) followed by 10 µM DEA/NO. Following treatment, cells were lysed and assayed for cGMP production. n = 3, * denotes P<0.05. <b>F</b> Porcine VSMC adhesion to collagen coated wells was assessed in the presence of Aβ (0.01–10 µM) for 1 hour. <b>G</b> Thrombin(0.2 U)-induced aggregation of washed human platelets was assessed in the absence (control) or presence of DEA/NO (0.01 µM) and with a 5 min pretreatment of Aβ (10 µM) followed by DEA/NO.