Flow cytometry detection and sorting of CXCR6+ subpopulations from cultures of human melanoma cell lines.

<p>Single marker CXCR6 analyses. Primary melanoma IGR39 cells and metastatic melanoma IGR37 cells were incubated with the indicated fluorescent APC-conjugated antibodies and analyzed by flow cytometry as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0015183#s4" target="_blank"><i>Materials and Methods</i></a>. <b>Y-axes</b>, side scatter; <b>x-axes</b>, relative fluorescence intensity. <b>Left panels</b>, analyses with isotype (IgG) control antibodies; <b>middle panels</b>, analyses with anti-CXCR6 APC-conjugated antibodies. After FACS isolation based on the indicated gate (<b>blue outline</b>), CXCR6+ sorted cells were reanalyzed to confirm enrichment (<b>right panels</b>).