Oxidative damage of DETC is restricted to amastigotes in the phagosome.

<p>Transmission electron microscopy of human macrophages infected with <i>Leishmania amazonensis</i>. Human macrophages were infected with <i>Leishmania amazonensis</i> (5∶1 ratio) for 4 h and then treated with DETC (2 mM) for 10 h. Untreated macrophages displayed numerous well-preserved parasites (A and B). DETC-treated macrophages presented remarkably damaged parasites (C and D), whereas host cell and parasite mitochondria were not injured (white and black arrows, respectively). P indicates intracellular parasites and N indicates host cell nucleus, bars represent 2 µm (A), 1 µm (B and C) or 0.5 µm (D). Images are representative of 2 independent experiments.