CD86 expression and viability of PBMDCs exposed to photoallergens and allergens with and without irradiation.

<p>PBMDCs were incubated with the indicated (X axis) concentrations of photoallergens and allergens with or without exposure to 1 J/cm² UVA. The ΔCD86 values (columns) and the cell viability (lines) were measured by flow cytometry without (open columns/squares) or with UVA irradiation (black columns/diamonds) after exposure to the following compounds: A: 6-methylcoumarin. B: musk ambrette. C: sparfloxacin. D: ketoprofen. E: olaquindox. F: α-hexylcinnamaldehyde. Mean values for cell viability and mean ± standard deviation for the ΔCD86 expression values measured on the PBMCDs from at least 5 independent donors are shown. Negative ΔCD86 values were due to biological variability. Asterisk indicates significant difference between irradiated and non-irradiated ΔCD86 values (p<0.05, Wilcoxon test).