Sags induce increases in the level of expression of Fas and in the percentage of Fas-L+ cells in lymphoma T cells.

<p>Lymphoma T cells display low levels of Fas expression. (A) Single cell suspensions from thymus from 2-5-mo-old animals (n = 8), 6-14-mo-old animals (n = 11) and spontaneous AKR/J thymic lymphomas (n = 28) were stained with anti-CD4, anti-CD8 and anti-Fas MoAbs. Data show the mean fluorescence intensity (MFI) of Fas in each group. Data are expressed as the mean ± SD. **p<0.01, ^† p<0.05, ^‡ p<0.05. (B, C, D, E) 2.5×10⁵ T5 or T8 lymphoma cells were co-cultured with 0.7×10⁵ intraperitoneal macrophages and treated with 10 µg/ml of SEI, SEB or PBS. At day 3, cells were harvested and stained with anti-Fas or anti-FasL MoAbs. (B, C) MFI of Fas, (D, E) percentage of FasL+ cells. Data are expressed as the mean ± SD. *p<0.05. (F, G) AKR/J mice were intraperitoneally inoculated with 5×10⁶ T8 CFSE- labeled cells and intraperitoneally injected with 25 µg of SEE (grey bar), SEB (black bar) or PBS (white bar). At days 1 and 2 cells were recovered, stained with anti-Fas or anti-FasL MoAbs and analyzed by FACS. Data are expressed as the mean ± SD, n = 4 per group. **p<0.01, *p<0.05. All the experiments were performed three times with similar results.