<p>10 nM KF or 20 nM HIV-1 RT were incubated with 250 nM poly-rA/dT<sub>18</sub> and 20 µM of a 3Dpol inhibitor (1A8, 4H6, 6B11, 5D9, 7F8, 8C5, or 9A3) in a buffer containing 50 mM Tris-HCl, pH 7.8, 60 mM KCl, 1 mM DTT, 0.01% BSA, 0.1% NP40, and 4% DMSO. DNA synthesis was initiated by the addition of 1 mM MnCl2 and 500 µM dTTP final concentrations. No significant inhibition of KF or HIV RT is observed under these conditions. Panel B shows the RNA synthesis by BVDV 3Dpol in presence of 40 µM of each of the compounds. The RNA synthesis by BVDV 3Dpol was primed by GG dinucleotide in 50 mM Tris-HCl pH 7.8, pH 7.8, 60 mM KCl, 1 mM DTT, 0.01% BSA, 1 mM MnCl2, 100 µM ATP, GTP, CTP and 10 µM UTP mixed with 5 µCi of α-32P-UTP. The extension of GG ca...
Several homologous polynucleotides have been tested as inhibitors on the reactions catalyzed by avia...
(E)-5-(2-Bromovinyl)-2'-deoxyuridine 5'-triphosphate (BrVdUTP) and (E)-5-(2-bromovinyl)-1-beta-D-ara...
BACKGROUND: Deoxynucleoside triphosphates (dNTPs) are the normal substrates for DNA synthesis cataly...
<p>Gel-based assays were used to evaluate the effect of K59A and K177A mutations on the susceptibili...
We tested three compounds for their ability to inhibit the RNase H (RH) and polymerase activities of...
<p>(A) Effect of inhibitors on binding of 3Dpol to template-primer (T/P). Binding of 3Dpol to fluore...
Moloney murine leukaemia virus (M-MuLV) is a member of the retrovirus family. Its cloned reverse tr...
Moloney murine leukaemia virus (M-MuLV) is a member of the retrovirus family. Its cloned reverse tr...
<p>Representative results depicting the dose-dependent inhibition of RNA synthesis by 5D9, 7F8, and ...
Some intercalating and nonintercalating drugs have been tested as inhibitorrs on the DNA synthesis r...
As α-carboxy nucleoside phosphonates (α-CNPs) have demonstrated a novel mode of action of HIV-1 reve...
Some intercalating and nonintercalating drugs have been tested as inhibitors on the DNA synthesis re...
The original publication is available at http://www.sajs.co.za/Moloney murine leukaemia virus M-MuLV...
Fragment-based screening methods can be used to discover novel active site or allosteric inhibitors ...
Background: Deoxynucleoside triphosphates (dNTPs) are the normal substrates for DNA synthesis cataly...
Several homologous polynucleotides have been tested as inhibitors on the reactions catalyzed by avia...
(E)-5-(2-Bromovinyl)-2'-deoxyuridine 5'-triphosphate (BrVdUTP) and (E)-5-(2-bromovinyl)-1-beta-D-ara...
BACKGROUND: Deoxynucleoside triphosphates (dNTPs) are the normal substrates for DNA synthesis cataly...
<p>Gel-based assays were used to evaluate the effect of K59A and K177A mutations on the susceptibili...
We tested three compounds for their ability to inhibit the RNase H (RH) and polymerase activities of...
<p>(A) Effect of inhibitors on binding of 3Dpol to template-primer (T/P). Binding of 3Dpol to fluore...
Moloney murine leukaemia virus (M-MuLV) is a member of the retrovirus family. Its cloned reverse tr...
Moloney murine leukaemia virus (M-MuLV) is a member of the retrovirus family. Its cloned reverse tr...
<p>Representative results depicting the dose-dependent inhibition of RNA synthesis by 5D9, 7F8, and ...
Some intercalating and nonintercalating drugs have been tested as inhibitorrs on the DNA synthesis r...
As α-carboxy nucleoside phosphonates (α-CNPs) have demonstrated a novel mode of action of HIV-1 reve...
Some intercalating and nonintercalating drugs have been tested as inhibitors on the DNA synthesis re...
The original publication is available at http://www.sajs.co.za/Moloney murine leukaemia virus M-MuLV...
Fragment-based screening methods can be used to discover novel active site or allosteric inhibitors ...
Background: Deoxynucleoside triphosphates (dNTPs) are the normal substrates for DNA synthesis cataly...
Several homologous polynucleotides have been tested as inhibitors on the reactions catalyzed by avia...
(E)-5-(2-Bromovinyl)-2'-deoxyuridine 5'-triphosphate (BrVdUTP) and (E)-5-(2-bromovinyl)-1-beta-D-ara...
BACKGROUND: Deoxynucleoside triphosphates (dNTPs) are the normal substrates for DNA synthesis cataly...