Inhibition of FMDV in cell-based assays.

<p>(A) Evaluation of anti-FMDV activity of 3Dpol inhibitors. Treatment with 15 µM of various compounds was performed following virus adsorption on BHK-21. The cells were further incubated in the presence of compound for another 24 hours and virus titer was determined by plaque assays (PFUs, plaque forming units) as described in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0015049#s4" target="_blank">Materials and Methods</a>. Experiments were done in duplicates. (B) Dose–dependence of FMDV inhibition by 5D9. Compound 5D9 was administered to BHK-21 cells in a dose-dependent manner prior to infection with FMDV. Post-infection, the compound was re-administered and incubated for another 24 hours. Following this incubation, samples were taken and plaque assays were performed. Results are reported as percent inhibition compared to a sample without inhibitor, and demonstrate a dose-dependent inhibition of virus replication. At the highest concentrations of 5D9 (20 µM) there was a greater than 90% inhibition of virus replication. Error bars represent standard error of the mean for two experiments. At these inhibitor concentrations no decrease in cell viability was observed.