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Comparative detection of H5N1-H05 HA protein expressed by AdHu5-HA and PAV3-HA.

  • Ami Patel (139999)
  • Suresh Tikoo (358706)
  • Gary Kobinger (294760)
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Publication date
February 2013
Icon DOI
10.1371/journal.pone.0015301.g001

Abstract

<p>Transgene expression was detected by Western Blot using polyclonal anti-H5N1 mouse sera and a goat anti-mouse-HRP secondary antibody. (A) M = Marker, Lane 1 = expression of AdHu5-HA in HEK 293 cells, Lane 2 = expression of PAV3-HA in VRIBLE1 cells, Lanes 3 and 4 expression of AdHu5-HA and PAV3-HA in mouse syngenic AB12 cells, (−) negative control, untransfected cell lysate. (B) <i>In vivo</i> expression in mouse muscle tissues 4 days following vaccination. 75 µg of muscle tissue was loaded in each lane to compare protein expression. M = Marker, Lanes 1–6 = PAV3-HA, Lanes 8–13 = AdHu5-HA, (−) = unvaccinated muscle tissue.</p

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