<p>After flow-through culture was complete, each pair of eyes was collected, RNA was extracted and real-time PCR was performed on GFP to compare relative levels of transgene expression. The average GFP levels from the XOP (n = 25) and CAR (n = 26) transgenic eyes were comparable and the difference was not statistically significant. Values are average relative GFP expression levels +/− SEM.</p
<p>(A) Absolute quantitative real-time PCR analysis of GFP copy number from newborn to maturity in f...
<p>GFP mRNA content in wild type females was measure by qRT-PCR. Each sample was obtained from eithe...
<p>L929 cell cultures infected with pASK-GFP-L2 transformed <i>C</i>. <i>trachomatis</i> were induce...
<p>A: Western blot using RPE/choroid protein extracts and GFP antibody. Positive transgenic mouse li...
<p>The expression values generated by RNA-seq (red) or qPCR (blu) for eleven selected genes using th...
<p>qPCR was performed on GFP as described in <a href="http://www.plosone.org/article/info:doi/10.137...
<p>(A) Relative real-time RT-PCR analysis of GFP mRNA expression from newborn to maturity in founder...
<p>Quantitative RT-PCR performed on mRNA harvested from KillerRed and GFP-injected eyes, three month...
Comparing expression patterns of CRISPR/Cas9-engineered reporter alleles with fosmid-based reporter ...
(A) Third instar eye imaginal disc expressing UAS-GFP.nls under ey-Gal4. Mean GFP fluorescence inten...
a<p>Average expression level in 3D skin equivalent calculated with the 2<sup>−ΔΔCT</sup> method.</p>...
<p>(a) Relative expression levels of <i>PAP1</i> or <i>Lc</i> in four transgenic <i>PAP1</i> callus ...
<p>(A) Count of up-and down-regulated genes in RAW 264.7 cells treated with MFP 100 μg/mL compared t...
<p>Relative expression levels of GFP were normalized to the expression of ribosomal protein 49 (<b><...
<p>GFP expression diverges between the transgenic lines at later developmental time points. Expressi...
<p>(A) Absolute quantitative real-time PCR analysis of GFP copy number from newborn to maturity in f...
<p>GFP mRNA content in wild type females was measure by qRT-PCR. Each sample was obtained from eithe...
<p>L929 cell cultures infected with pASK-GFP-L2 transformed <i>C</i>. <i>trachomatis</i> were induce...
<p>A: Western blot using RPE/choroid protein extracts and GFP antibody. Positive transgenic mouse li...
<p>The expression values generated by RNA-seq (red) or qPCR (blu) for eleven selected genes using th...
<p>qPCR was performed on GFP as described in <a href="http://www.plosone.org/article/info:doi/10.137...
<p>(A) Relative real-time RT-PCR analysis of GFP mRNA expression from newborn to maturity in founder...
<p>Quantitative RT-PCR performed on mRNA harvested from KillerRed and GFP-injected eyes, three month...
Comparing expression patterns of CRISPR/Cas9-engineered reporter alleles with fosmid-based reporter ...
(A) Third instar eye imaginal disc expressing UAS-GFP.nls under ey-Gal4. Mean GFP fluorescence inten...
a<p>Average expression level in 3D skin equivalent calculated with the 2<sup>−ΔΔCT</sup> method.</p>...
<p>(a) Relative expression levels of <i>PAP1</i> or <i>Lc</i> in four transgenic <i>PAP1</i> callus ...
<p>(A) Count of up-and down-regulated genes in RAW 264.7 cells treated with MFP 100 μg/mL compared t...
<p>Relative expression levels of GFP were normalized to the expression of ribosomal protein 49 (<b><...
<p>GFP expression diverges between the transgenic lines at later developmental time points. Expressi...
<p>(A) Absolute quantitative real-time PCR analysis of GFP copy number from newborn to maturity in f...
<p>GFP mRNA content in wild type females was measure by qRT-PCR. Each sample was obtained from eithe...
<p>L929 cell cultures infected with pASK-GFP-L2 transformed <i>C</i>. <i>trachomatis</i> were induce...