Doxorubicin induces cell cycle alterations and impairs cell viability.

<p>(A) Cell cycle analysis: the blue area represents the control cells, the red line indicates treated cells. Cells were cultured for 24 h after treatment with doxorubicin 0,25 µM and then analyzed. Phases of the cell cycle are indicated: Sub G0 [M1], G0/G1 [M2], S [M3], G2/M [M4], >4N [M5]. (B) Cell proliferation evaluated by BrdU incorporation. Cells were cultured for 24 h after treatment with various doses of doxorubicin and then analyzed. BrdU data were normalized to untreated (media alone) cells (C), Number of viable cells evaluated by MTT assay. Cells were analyzed before treatment and 24 hours after treatment with various doses doxorubicin. ct, control; Dox, doxorubicin. *p<0.05 vs ct. § p<0.05 vs 0 h; # p<0.05 vs ct 24 hr.