Binding and inhibition of the binding of β2GPI in normal human serum to cardiolipin.

<p>A) The binding of β2GPI in serum to cardiolipin-coated surface in the absence (triangles) and in the presence (circles) of anti-β2GPI antibodies. B) Inhibition of the binding of β2GPI in human serum to cardiolipin in the presence of anti-β2GPI antibodies by the dimeric inhibitor A1-A1 (circles) and monomeric inhibitor A1 (triangles). Fit to one-site binding and inhibition models was performed on the raw data. To facilitate comparison, the measured OD₄₀₅ values and the binding curves were normalized to the maximum binding obtained from the fit.