Dominance of CD137 protein-induced monocytic differentiation.

<p>5×10⁵ lin⁻, c-kit⁺ cells at a density of 5×10⁵ cells/ml were cultured on plates that had been coated with 10 µg/ml of Fc or CD137-Fc protein or that were not coated (PBS, medium control). Where indicated, G-CSF was added daily at 10 ng/ml. The percentages (A) and absolute numbers (B) of CD11b⁺, Ly6G⁻ and CD11b⁺, Ly6G⁺ cells, and the percentages (C) and absolute numbers (D) of CD115⁺, MPO⁻ and CD115⁻, MPO⁺ cells were determined by flow cytometry on day 7. (A) and (C) are representatives of five and two experiments, respectively, (B) and (D) are depicted as means ± standard errors of five and two independent experiments, respectively. * p<0.05.