Effects of ds<i>Cq-IAG</i> injections on maternal care-related characteristics in intersex crayfish.

<p>Pleopods were collected from intersex animals injected with DDW (A) or ds<i>Cq-IAG</i> (B) and from mature females (C). Length:width ratios between endopods and exopods of DDW-injected intersex were identical (A), while those of dsRNA-injected animals (B) showed female-like biometrics. Whereas the inner side of the endopod of DDW-injected intersex bore only plumose setae (D), as in males, the inner side of the endopod of dsRNA-injected animals was lined with ovigerous simple setae (E) as is the case of mature females (F). Bottom row (bar = 100 µm) represents an enlargement of the areas defined in squares in the top row (bar = 500 µm).