Involvement of CFTR in mediating cAMP-stimulated bicarbonate secretion by rat prostate epithelial cells.

<p>(A) The pH_i recovered quickly after cellular alkalinization induced by removing bicarbonate/CO₂ from perfusate in the presence of Cl⁻. (B) The rate of pH_i recovery was markedly attenuated when extracellular Cl⁻ was removed from the perfusate. (C) In the absence of Cl⁻, forskolin (forsk, 10 µM) stimulated pH_i recovery. (D) The forskolin-induced pHi recovery could be blocked by NPPB (100 µM). The scales in A–D are the same. (E) Summary of pH_i recovery rates under different conditions after cellular alkalinization induced by removing bicarbonate/CO₂ from perfusate. (*P<0.05).