FACS analysis of intact Env-expressing cells demonstrates extracellular exposure of the KE sequence.

<p>Cells transfected with HIV-1 89.6 WT, VSV-G-KE, or VSV-G-LLP2 were analyzed by FACS to determine VSV-G epitope accessibility to anti-VSV-G MAb. A.) Intact, non-permeabilized Env-expressing cells were stained with α-VSV-G (AlexaFluor (AF) 700) and α-actin (AF 488). B.) Intact cells expressing Env were stained for surface exposure of the KE using a native KE antibody, SAR1. C.) Same as (B) except cells were permeabilized prior to staining.