Analysis of expression of <i>VdGARP1</i> mRNA and formation of melanized microsclerotia under near-UV light.

<p>(A) Mycelial growth and melanized microsclerotial production under normal dark-grown conditions and near-UV light, photos were taken at 14 days post-culture on PDA agar medium. (B) RNA gel blot analysis of <i>VdGARP1</i> mRNA expression of V592 on PDA agar medium (left panels) and time course from liquid culture (right panels) exposure to near-UV light. rRNAs stained with methylene blue trihydrate were used as a loading control. Quantification of VdGARP1 mRNA relative to total RNA is shown below of the panel. The value of 0 hour treatment was arbitrarily designated as 1. (C) Development of melanized microsclerotia of V592 in infertile nutrient conditions. Photographs were taken at 4-, 5- and 6-dpc. (D, E) Melanized microsclerotial production of V592 and the <i>vdgarp1</i> mutant spores were grown in either liquid Czapek-Dox medium or infertile nutrient agar medium. Melanized microsclerotia under 400x microscope (E).