MSC express Sca-1 and CD44 and are capable of differentiating into adipocytes and osteocytes.

<p>A: WT and TLR4KO MSC were incubated with specific surface marker antibodies or isotype control antibodies and subjected to flow cytometry analysis. B: MSC in both genotypes were cultured with induction media for 3–4 weeks to induce cell differentiation. The expression of FABP-4 (adipocyte-marker) or osteopontin (osteocyte-marker) was examined using a florescence microscopy (NL557-positive red staining). Representative fields are shown at 100× magnification.