dsRNA effect on <i>Le. major</i> development.

<p>Intra-thoracic injections of dsPpChit1 (80.5 ng) reduce <i>Le. major</i> load in <i>P. papatasi</i> midgut. (A). At 48 h PBM, <i>Le. major</i> density was reduced on average 46% in dsPpChit1 (dsChit1) injected compared with dsControl (dsCtr) injected. (B). <i>Le. major</i> parasites per midgut were further reduced at 120 h PBM in dsPpChit1 injected flies, reaching on average 63% reduction over the dsControl injected ones. Each dot represents parasite number in a single <i>P. papatasi</i> midgut. Horizontal bars display mean parasite numbers. n: Number of flies analyzed. *: Statistically significant at p<0.05. Graphs represent one similar result of two independent experiments.