<p>(A) Wild-type, (B) heterozygous and (C) homozygous fibroblasts immune-stained for fibrillin-1 (scale bars 50 µm). In contrast to the elaborate network of immunoreactive fibrillin-1 seen in control lines, heterozygous and homozygous cell cultures show a diffuse pattern of immunoreactive material. (D) Representative field at higher magnification to show intracellular deposition of mutant protein (scale bar 25 µm).</p
Summary. Antibodies against intermediate-sized filaments, of the prekeratin or vimentin type, were u...
<p>FIBA and derivatives are used as examples. <b>A–C</b>) Phase-contrast images showing the morpholo...
<p>Primary cardiac fibroblasts were cultured overnight in 3D fibrin gels with points of tension prov...
<p><b>A,</b> MHC class I and class II expression in fetal cells. Flow cytometry analysis of MHC clas...
<p>(A) AS fibrils formation of (blue circles) E46K, (red triangles) A53T and (black squares) WT moni...
<p>Fibroblasts were stained with antibodies to Fibroblast Surface Protein (FSP), FVIII, and VWF plus...
A. Indirect immunofluorescence with anti-fibrillin-2 and anti-caveolin-1 antibodies labelling CAV1-/...
<p>Fibroblast lines were immunostained with antibodies FSP1 and TE7 at multiple consecutive passages...
<p>Primary human lung myofibroblast cultures between passages 4 and 5 were stained with myofibroblas...
<p>A: Fibroblasts developed a characteristic bipolar spindle-shape at 7 days after seeding (100×magn...
<p>Immunofluorescence staining in cultured MLG cells using antibodies to lactoferrin (Row A), E-cad ...
<p>(A) The morphology of wild type (WT) and TEF (−/−) fibroblasts in culture was assessed by phase c...
<p>The indicated cells were fixed, permeabilized and stained for immunofluorescence microscopy using...
<p>(A, C) CFF cells transiently transfected with pDG-miRNA plasmid in a fluorescent field. (B) Cells...
<p>(A) Cells freshly isolated from 22 week fetal myocardium were cultured for 2 days and then immuno...
Summary. Antibodies against intermediate-sized filaments, of the prekeratin or vimentin type, were u...
<p>FIBA and derivatives are used as examples. <b>A–C</b>) Phase-contrast images showing the morpholo...
<p>Primary cardiac fibroblasts were cultured overnight in 3D fibrin gels with points of tension prov...
<p><b>A,</b> MHC class I and class II expression in fetal cells. Flow cytometry analysis of MHC clas...
<p>(A) AS fibrils formation of (blue circles) E46K, (red triangles) A53T and (black squares) WT moni...
<p>Fibroblasts were stained with antibodies to Fibroblast Surface Protein (FSP), FVIII, and VWF plus...
A. Indirect immunofluorescence with anti-fibrillin-2 and anti-caveolin-1 antibodies labelling CAV1-/...
<p>Fibroblast lines were immunostained with antibodies FSP1 and TE7 at multiple consecutive passages...
<p>Primary human lung myofibroblast cultures between passages 4 and 5 were stained with myofibroblas...
<p>A: Fibroblasts developed a characteristic bipolar spindle-shape at 7 days after seeding (100×magn...
<p>Immunofluorescence staining in cultured MLG cells using antibodies to lactoferrin (Row A), E-cad ...
<p>(A) The morphology of wild type (WT) and TEF (−/−) fibroblasts in culture was assessed by phase c...
<p>The indicated cells were fixed, permeabilized and stained for immunofluorescence microscopy using...
<p>(A, C) CFF cells transiently transfected with pDG-miRNA plasmid in a fluorescent field. (B) Cells...
<p>(A) Cells freshly isolated from 22 week fetal myocardium were cultured for 2 days and then immuno...
Summary. Antibodies against intermediate-sized filaments, of the prekeratin or vimentin type, were u...
<p>FIBA and derivatives are used as examples. <b>A–C</b>) Phase-contrast images showing the morpholo...
<p>Primary cardiac fibroblasts were cultured overnight in 3D fibrin gels with points of tension prov...