TLR4KO MSC ablated with STAT3 produced lower levels of VEGF, HGF and IGF-1 compared with TLR4KO MSC ablated with GAPDH.

<p>TLR4KO MSC were plated in 12-well plates at 0.5×10⁵cells·well⁻¹·ml⁻¹24 h prior to siRNA transfection. The lipofectamine-siRNA complex (100 nM) was then added on culture day 2. Medium was then changed after 2 days (day 4 in culture) of transfection. 3 days after transfection (day 5 in culture), the supernatant was collected from TLR4KO MSC either transfected with STAT3 siRNA or GAPDH siRNA and was analyzed by using VEGF, HGF and IGF-1 ELISA. Total amounts of VEGF (A), HGF (B) and IGF-1(C) were examined. siRNA targeting of STAT3 did not change the proliferation of TLR4KO cells (D). Data are representative of at least three independent experiments. Results are mean ± SEM; n = 3/group. * <i>p</i><0.05 vs. GAPDH ablated group.