Secondary CTL response in mice immunized with OVA_257-264-liposome.

<p>Mice were immunized with 50 µl of OVA_257-264-liposome in the presence of 5 µg CpG, and 2, 4, 8, 16, and 20 weeks later, they received a booster ip injection with 200 µl of 1 mg/ml OVA in PBS (closed box) or no booster injection (open box). Three days after the booster injection, <i>in vivo</i> CTL assay was performed. Data represent mean percentages of cells killed and SEs of three mice per group. ND, not detected. *, significant difference (p>0.01).