Dose-Response of TGF-β1 on C2C12 Proliferation and Differentiation.

<p>C2C12 cells were treated with different concentrations of TGF-β1 for 96 hours and stained for nuclei and MHC. (A) Cell proliferation, as denoted by number of nuclei, increased in a dose-dependent manner with an EC50 for TGF-β1 of 17 pM. (B) Myoblast differentiation, as measured by percentage of nuclei positive for MHC, decreased with increasing concentrations of TGF-β1 with an IC50 of 23 pM.