Decreased MeCP2 expression in tTA::miR132 mice.

<p>A and B: Top panels) Representative immunohistochemical labeling for MeCP2 in the dorsal hippocampus of tTA monotransgenic (A) and tTA::miR132 bitransgenic (B) mice. Relative to tTA littermates, mice over-expressing miR132 showed a decrease in MeCP2 expression within the excitatory cell layers of the hippocampus. Scale bar: 200 µm. Middle and bottom panels) High magnification images of MeCP2 labeling within area CA1 and the GCL are presented. Scale bar: 100 µm. (C) Quantitative analysis of the mean ± SEM MeCP2 expression in area CA1 and the GCL. Of note, MeCP2 expression was significantly reduced in tTA::miR132 mice (n = 7) compared to monotransgenic controls (n = 8) (**P<0.01, *P<0.05). (D) Western analysis of biological replicates (two animals per condition) confirming the reduction in MeCP2 expression in tTA::miR132 mice, along with quantitative densitometric analysis relative to endogenous ERK1 levels (E), presented as mean ± SEM (n = 3) (*P<0.05).