Functionally cloned genes are neuroprotective.

<p>(A and B). Primary cortical neurons were transfected with 28 of the identified genes or the empty pCI vector or the ADCGI vector. EGFP serves as the reporter for transfected cells. 48 h later, the neurons were treated with (A) OGD (90 min) or (B) NMDA (500 µM, 5 min). 24 h after treatment, neurons were stained with Hoechst 33342 and propidium iodide. Only EGFP positive cells were scored. Dead neurons were scored as those cells that were propidium iodide positive, with condensed, or fragmented nuclei. Experiments were performed at least three times. Data are the mean ± SEM. *<i>p</i><0.01 (Student's <i>t</i>-test) comparing empty vector to each identified gene.