<i>her4::gfp</i> transactivation and premature differentiation of neural cells in <i>asb11^cul</i>.

<p>(<b>A</b>), the <i>her4::gfp</i> reporter was co-injected with <i>myc-tag</i> (MT) mRNA as a control, myc-tagged <i>d-asb11</i> full length (MT-Asb11) or myc-tagged <i>asb11^cul</i> (MT-Asb11^cul) mRNA in zebrafish embryos. Injected embryos were treated with (+) (n = 25) or without (−) (n = 25) DAPT, from 1.5 hpf. At 14 hpf, embryos were analyzed for <i>her4</i> transactivation based on the intensity of the GFP signal. Positive embryos were counted and percentages of embryos presenting weak (blue), medium (green) or strong (red) signal were given. (<b>B</b>), Wild type (<i>left panel</i>) and mutant (<i>middle panel</i>) embryos at 12 hpf were analyzed for WISH using probe against <i>ngn1</i>. (<i>right</i>) Graph quantifies expression of <i>ngn1</i> using qPCR. (<b>C</b>) Wild type (<i>left panel</i>) and mutant (<i>right panel</i>) polster of embryos at 16 hpf were analyzed for WISH using probe against <i>islet1</i>.