Gene expression in wild-type and LXR-deficient tissues and macrophages following Leishmania infection.

<p>A. Wild-type and LXRα-deficient mice were intravenously challenged with 1×10⁷ live, stationary-phase <i>L. chagasi/infantum</i> parasites, and RNA was prepared from livers and spleens 24 and 72 hours following infection. Real-time RT-PCR was performed with SYBR primers specific for LXRα and LXRβ. B. BMDM were preincubated overnight with IFN-γ, infected with stationary-phase <i>Leishmania</i> for 1 hour, washed to remove extracellular parasites, and incubated for an additional 2 hours. RNA was harvested, and levels of various genes were determined by real-time RT-PCR. Error bars represent standard deviations of replicate infections; ** denotes p<0.005. Results are representative of at least 3 independent experiments.