Cytokine expression profile of <i>S</i>.Tm^*-infected <i>Il17ra^+/−</i>, <i>Il17ra^−/−</i>and C57BL/6 mice.

<p><i>Salmonella</i>-infected animals (n = 3) from experiment shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0013804#pone-0013804-g002" target="_blank">Fig. 2</a> were used for analysis of gene expression signature. Cecum samples from <i>Il17ra^−/−, Il17ra^+/−,</i> and C57BL/6 mice 12 h p.i. with <i>S</i>.Tm*, <i>S</i>.Tm^SipA or <i>S</i>.Tm^SopE, were taken for RNA isolation and RT-PCR (B–D). C57BL/6 mice, only treated with streptomycin, were used as a control, not differing significantly from <i>Il17ra^−/−</i> (A). 45 genes related to inflammation and defense, especially in IL-17-mediated responses were used. The analysis was performed using RealTime StatMiner Software; mRNA levels were normalized to 3 house-keeping genes: <i>Actb, Gapdh</i> and <i>18S</i> (ΔCt). Relative quantification was determined by the ΔΔCt method after normalization to streptomycin-treated C57BL/6 animals. Statistical analysis was done using the Limma test with Bonferroni correction (B). All results for <i>S</i>.Tm^*-infection did not differ significantly between <i>Il17ra^−/−</i> and <i>Il17ra^+/−</i> mice (p≥0.05).