Cullin box is essential for DeltaA degradation and for maintaining a cell proliferating state <i>in vivo</i>.

<p>(<b>A</b>) Zebrafish embryos were injected with Myc-tagged <i>deltaA</i> (MT-DeltaA) and <i>d-asb11</i> (Asb11) or <i>asb11^cul</i> (Asb11^cul) mRNA at one-cell stage. (<i>lower panel)</i> Lysates of 12 hpf embryos were analyzed by immunoblotting for the presence of DeltaA. (higher panel) Graph quantifies 2 individual experiments, each with 30 injected embryos/group. (<b>B</b>), Fluorescent whole-mount antibody labeling of wild type (WT) and <i>asb11^cul</i> embryos at 24 hpf for the mitotic marker anti-phosphohistone-3 (PH 3) antibody (<i>green</i>) and the neuronal marker Hu(C). Graph shows the number of positive cells per area (5 somites from beginning of yolk extension) of 5 embryos for each genotype.