<i>Il17ra</i> expression in the gut of control and <i>S</i>.Tm^*-infected C57BL/6 mice.

<p>(A) <i>Il17a</i>, <i>Il17f</i> and <i>Il17ra</i> expression in the intestinal tissues of naïve mice (n = 3 per group). Relative levels of mRNA in 20 ng total RNA were normalized to <i>house keeping genes</i>. ΔCt values do not strictly correlate to the difference in gene expression between various genes. However, ΔCt values <19 represent detectable levels of expression, demonstrating thereby that all three genes are expressed in the various tissue segments. (B) Induction of <i>Il17ra</i> expression in <i>Salmonella</i>-infected animals. <i>Salmonella</i>-infected animals (n = 3; 12 h p.i. with <i>S</i>.Tm*; open circles) were from the experiment shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0013804#pone-0013804-g002" target="_blank">Fig. 2</a>. Non-infected, stereptomycin pretreated C57BL/6 mice (n = 3; black circles) served as a contol. Samples of gut tissues (A, as indicated; B, cecum tissue) were taken for RNA isolation and mRNA levels were analyzed by real time PCR. <i>S</i>.Tm^* infected C57BL/6 mice showed a significant up-regulation of <i>Il17ra</i> in comparision to non-infected C57BL/6 mice (p<0.05; t-test unpaired, two-tailed). Sm: Streptomycin. Line: median.