Aldehyde dehydrogenase (ALDH) expression in ESFT cell lines.

<p>The indicated cell lines were treated with Aldefluor reagent alone (top) or in the presence of the ALDH inhibitor DEAB (bottom), and then analyzed by FACS. Each cell line has an Aldefluor bright cell population that is undetectable in the presence of DEAB. In each panel, the box indicates a typical gate, containing approximately 2% of the cells, defined as ALDH^high and used for subsequent experiments. Of note, these cells also have low side scatter. These sorting experiments have been performed a minimum of three times on each cell line.