Preclinical detection of PrP^Sc in muscle of vCJD inoculated primates.

<p>A) Western Blot analysis of PrP^Sc from NaPTA precipitated arm and tongue tissue of sCJD, vCJD and BSE infected primates and controls show PrP^Sc in arm and tongue of subclinical vCJD challenged monkeys and in clinically affected vCJD and BSE inoculated animals. sCJD infected macaques did not show any detectable PrP^Sc in arm or tongue. B) Detection of PrP^Sc in arm, tongue and heart tissue from sCJD, vCJD and BSE infected primates and controls by ELISA. The scatter graph shows values (mean of two independent experiments) for arm (circle), tongue (triangle) and heart (diamond). The cut-off is indicated by grey shade and values below were considered as negative. PrP^Sc could be detected in tongue, arm and heart of healthy and clinically diseased vCJD inoculated animals. In BSE infected animals PrP^Sc could only be detected in tongue tissue and in sCJD infected animals no PrP^Sc could be detected.