PTK7 expression and cell apoptosis induced by knocking down of PTK7 in p53-null HCT 116 cells.

<p>(A) Flow cytometry assay for the binding of the PE-labeled anti-PTK7 with p53-null HCT 116 cells (Grey curves). The black curves represent the background binding of anti-IgG-PE. The concentration of the antibody in the binding buffer was 2 µg/µL. (B) The number of live p53-null HCT 116 cells was counted on day 4 after treatment with vehicle, nonspecific siRNA and PTK7 siRNA. Data are mean±s.d. of three independent experiments. *Student's t-test: P<0.05. (C) BrdU incorporation relative to untreated cells detected by flow cytometry. p53-null HCT 116 Cells were incubated with 10 µM BrdU for 2 h after 48 h of treatment. The amount of BrdU incorporation was normalized to the untreated group. Data are mean±s.d. of three independent experiments. *Student's t-test: P<0.05. (D) Apoptosis occurrence in p53-null HCT 116 cells detected by Annexin V/PI stain on days 4 after transfection. Cells stained negative for both Annexin V and PI were considered healthy and percentage was shown in the figure.