Na/K pump subunits distribution upon subcellular fractionation of CsA-treated or CypB-silenced HK-2 cells.

<p>Sh Luc, Sh Luc treated with CsA, and Sh CypB cells show different patterns of sedimentation on sucrose gradients of α and β Na/K ATPase and CypB. Cells treated or untreated with CsA were harvested and loaded on sucrose gradients (10 to 50% w/v) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0013930#s4" target="_blank">Methods</a>. (A) Western Blot of fractions eluted from sucrose gradient of Sh Luc cells probed against protein markers of specific cell compartments: Syntaxin-6 (Golgi), Calnexin (ER), E-cadherin (Plasmatic Membrane) and S6 (Ribosomes). (B) Western Blot of fractions eluted from sucrose gradient of Sh Luc, Sh Luc + CsA, and Sh CypB cells probed against α and β Na/K ATPase and CypB. Figures are representative of at least three independent experiments.