Subcellular localization of ER-α36 and ER-α66 in Ishikawa cells.

<p>A. Ishikawa cells cultured on coverslips were fixed and immunofluorescently stained with the anti-ER-α36 specific antibody (green). The cells were also stained with Hoechst 33258 (blue) to show the cell nuclei. B. ER-α66 expression detected by immunofluorescence in Ishikawa cells. The nucleus was stained by Hoechst 33258. Bar, 10 micrometers.