Expression level of MTA1 in different segments of adult mouse epididymis.

<p>(A) The products of a representative semi-quantitative RT-PCR were subjected to electrophoresis on a 1.5% agarose gel. <i>GAPDH</i> was used as an internal control for each PCR amplification. (B) Relative expression levels in RT-PCR were obtained in each sample by normalization of absolute optical densities (ODs) of the specific target to that of the <i>GAPDH</i> signal. (C) Western analysis of MTA1 protein in total tissue protein extracts from caput, corpus and cauda of the epididymis and testis. The blot was reprobed with a β-actin (42 kDa) monoclonal antibody to confirm equal loading of proteins in all lanes. (D) Semi-quantitative values are normalized to those of loading controls (β-actin) to express arbitrary units of relative expression. Comparison of the relative densities between groups in both assays was performed by ANOVA. (* p<0.05 and ** p<0.01).