Activation of <i>L. (L.) amazonensis</i> and <i>L</i>. (<i>L</i>.) <i>chagasi</i> infected macrophages.

<p>Infected macrophages were cultured in the presence of TNF-α plus LPS. Four days later, cultures were fixed and stained for infection index determination. <i>L</i>. (<i>L</i>.) <i>chagasi</i> was used as a positive control for activation (A). Nitric oxide was assayed in the culture supernatants (B). Bars represent the SD. ^*<i>P</i><0.001.