Expression level of MTA1 in mouse epididymis following castration and subsequent testosterone manipulation.

<p>(A) RT-PCR assay of adult mouse epididymis RNA from precastration (C0); bilaterally castrated for 1, 3, 5, and 7 d (C1, C3, C5, C7) and 1, 3, 5 and 7d after a single injection of testosterone propionate applied to the 7-d castrated mice (C7+1, C7+3, C7+5 and C7+7). The total RNAs were pooled from six animals at each time-point. (B) Western blot analysis of MTA1 and β-actin proteins from adult mouse epididymis at different time points as described above. (C) The relative expression levels of <i>MTA1</i> mRNA (expression density of <i>MTA1</i> mRNA/<i>GAPDH</i> mRNA) and MTA1 proten (expression density of MTA1/β-actin) in the mouse epididymis were compared with the serum testosterone level (expressed in nanomoles per liter) during androgen manipulation (n = 6).