Inflammatory response to MHV-68 in MEFs is dependent on TLR2 expression.

<p>(<b>A</b>) WT and TLR2−/− MEFs were stimulated with mock, infectious MHV-68 at indicated m.o.i. or LPS (1 µg/ml) for 18 hours. Cell-free supernatants were then assessed for IL-6 secretion by ELISA assay. (<b>B</b>) WT and TLR2−/− MEFs were stimulated with mock or infectious MHV-68 (m.o.i. of 1) for indicated periods of time or with LPS (1 µg/ml, 18 hours). Cell-free supernatants were then assessed for IL-6 secretion. (<b>C</b>) WT and TLR2−/− MEFs were stimulated with mock, infectious MHV-68, UV-irradiated MHV-68 (m.o.i. of 1) or LPS (1 µg/ml) for 24 hours and cell-free supernatants were then assessed for IL-6 secretion. (<b>D</b>) WT, TLR2−/− and MyD88−/− MEFs were stimulated with mock or infectious MHV-68 (m.o.i. of 1) for 24 hours and 48 hours or with LPS (1 µg/ml) or Pam₃CSK₄ (Pam, 1 µg/ml) for 48 hours and cell-free supernatants were then assessed for IL-6 secretion. (<b>E</b>) WT, TLR2−/−, MyD88−/− and TRIF−/− MEFs were stimulated with mock or with infectious MHV-68 (m.o.i. of 1), LTA (10 µg/ml) or poly(I:C) (5 µg/ml, transfected with Lipofectamine) for 24 hours. Levels of IFN-α were then determined in cell-free supernatants by ELISA. Data are representative of three different experiments. *<i>p</i><0.05.