Positive selection for CD8 Tg T cells was partially reduced in <i>Foxn1^Δ/Δ</i> mice.

<p><i>Foxn1</i>^Δ/Δ;OT1 and <i>Foxn1</i>^Δ<i>/</i>Δ;OT1 Tg mice were generated by mating <i>Foxn1</i>^Δ/Δ mice with OT1 CD8 TCR Tg mice, and the CD8 Tg cells were analyzed in thymocytes (A, B, C and D) and splenocytes (E, F, G and H). A, representative profile of CD4 and CD8 expression on thymocytes (top panel), and expression of Vα2 and Vβ5 on gated CD8 SP thymocytes (bottom panel). B, percentage of CD8 SP thymocytes in individual OT1 Tg and non-Tg mice; C, individual analysis of the percentage of Vα2⁺ and Vβ5⁺ cell on gated CD8 SP thymocytes; D, total number of thymic cells from individual OT1 Tg and non-Tg mice. E, F, G and H show the same analyses performed on splenotytes. (*, p<0.05).