Effect of <i>cis</i>-UCA and DSS on tissue cytokines.

<p>129Sv/Ev mice were treated with 5% DSS for 7 days to induce acute colitis. Beginning on the first day of DSS administration mice were injected subcutaneously along the dorsal axis daily for 7 days with 50 µg <i>cis</i>-UCA or vehicle (PBS). Colonic tissue was ultrasonicated and supernatants were analyzed for total levels of cytokines. <i>cis</i>-UCA and DSS had no significant effects on tissue levels of IL-10 (A). DSS treated mice had increased levels of IL-17, and these were further increased in mice treated with <i>cis</i>-UCA (50 µg) (B). DSS treated mice had increased levels of colonic IL-6 (C) and IL-23 (D) and these values were not altered by <i>cis</i>-UCA (50 µg) n = 5–25 for all measurements. a: p<0.01 DSS+PBS compared with control PBS, control UCA (50 µg), and DSS+UCA (50 µg). b: p<0.01 DSS+UCA (50 µg) compared with control PBS and control UCA (50 µg). c: p<0.01 DSS+PBS compared with control PBS, and control UCA (50 µg). d: p<0.01 DSS+UCA (50 µg) compared with control PBS, and control UCA (50 µg). e: p<0.01 DSS+PBS compared with control PBS, and control UCA (50 µg). f: p<0.01 DSS+UCA (50 µg) compared with control PBS, and control UCA (50 µg).