Virus load reduction is not enhanced by a second dosing with Ab.

<p>BALB/c mice were given 500 pfu of PR8 virus in 10 µl by the intranasal route to establish a localised URT infection. One and 3 days post-infection, mice were treated with 200 µg of each Ab preparation or PBS via the URT route. Five days post-infection, mice were killed and nasal turbinates were collected in 1 mL media. Viral titers were determined by plaque assay on MDCK cell monolayers. The experiment was performed once with 4 mice per group, the organs assayed in quadruplicate and titers averaged to provide a value for that animal. Each symbol represents an individual mouse and the line represents the mean of each group. Brackets with an asterisk indicate a statistically significant difference between the indicated groups.