Distribution patterns of endosomes in RNAi pollen tubes were altered after down-regulating <i>NtGNL1</i> Expression.

<p>A and D are bright field images; others are fluorescent images or schematic diagrams. <b>A–C:</b> Control, showing normal fluorescence distribution in a converse V-shape. <b>D–F:</b> RNAi pollen tube showing fluorescence accumulated at the sub-apical region. The arrows indicate the direction of the vesicle movement. Images were taken between 30 and 60 min after loading FM4-64 to 3 h-cultured pollen tubes. Bar = 10 µm.