The life span of the <i>Da-GAL4>UAS-TG IR</i> flies.

<p>(A) The life span of the RNAi flies was compared with those of the control flies, <i>Da-GAL4>+</i>, <i>Da-GAL4>UAS-lacZ IR</i> and <i>+>UAS-TG IR</i>. Sixty adult flies were collected and maintained at 25°C. The number of surviving flies was recorded daily. The means ± S. D. of four independent experiments were plotted. <i>Da</i>, <i>Da-GAL4</i>. (B) Phenotypes of <i>Tub-GAL80^ts; Da-GAL4>UAS-TG IR</i>. <i>Tub-GAL80^ts; Da-GAL4</i> flies were crossed with the <i>UAS-TG IR</i> flies in 20 vials and maintained at 18°C. The suppression of <i>TG</i> by RNAi was triggered by increasing the temperature to 29°C. The ratios flies with abnormal wings (square) and abnormal abdominal cuticles (circle) to total adult flies are indicated (upper panel). The number of adult flies born from each vial is indicated (lower panel).