Chemokine receptor expression profile on MDC and PDCs in patients with PDAC.

<p>Blood MDCs (HLA DR⁺CD11c⁺Lin⁻) and PDCs (HLA DR⁺CD123⁺Lin⁻) from PDAC patients pre (one week) and post (12 weeks) surgery were compared to age matched controls. The DC subsets from each group were stained using direct conjugated antibodies against CCR2 (A), CCR5 (B), CCR6 (C), and CCR7 (D) and detected by multi-color flow cytometry. Mean fluorescence intensity (MFI) values or the amount positive MDCs and PDCs in percentage were compared between the different groups using nonparametric Wilcoxon signed rank test used for paired data and Mann–Whitney test for calculation of p values. Statistically significant differences between individuals with PDAC and healthy controls are indicated as; * = p<0.05, ** = p<0.005, *** = p<0.001.