F-actin disorganization in <i>NtGNL1</i> RNAi pollen tubes.

<p>A and H: wild type pollen tubes. Others are RANi pollen tubes. <b>A</b>: Wild-type pollen tubes show no straight F-actin bundles at the clear zone. <b>B</b>: Twisted bundles extend to the clear zone of pollen tip(arrow). <b>C:</b> Condensed F-actin bundles (lower arrow) abundantly extend to the tip region of pollen tube (upper arrow). <b>D</b>: Thick bundles accumulate in expanded pollen tube tip and extend perpendicular to the tube long axis. <b>E</b>: Disorganized actin bundles accumulated at the pollen tip. <b>F</b>: Thick and twisted actin bundles bending in RNAi pollen tube. <b>G</b>: Actin fine fringe perpendicular to the plasma membrane (arrow) and absent at the clear zone in control. <b>H</b>: actin fringes collapsed at the sub-region and shifted to the clear zone (arrow). A- F. revealed by LAT52-ABD2-GFP; G and H were labeled by FITC–phalloidin. Bar = 20 µm in A- F. Bar = 10 µm in G and H.